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Biology Senior High

問3.4が分かりません詳しくだけると幸いです

抗生物質は大腸菌などの細菌の増殖を防ぐために用いられる物質で、遺伝子組換え実験にも な 利用される。 以下の実験で用いたプラスミドは図1に示すように、抗生物質であるアンビシリンの作用を 抑える遺伝子(Amp))とラクトース(乳糖)を分解する酵素であるラクターゼの遺伝子(LacZ)を このグラスミドに特定の塩基配列を認識して切断する酵素(A) を作用させて切断した(図1)。 また、同じ酵素を用いてヒトのDNAから遺伝子Xを含む DNA 断片を切り出した(図2)。 素(A)による切断の模式図を図3に示す。 大陽室の両者を混合し、切断点をつなぐ別の酵素(B)を作用させた(図4)。この混合液を特殊な処理 あみをした大腸菌と混ぜたところ、一部の大腸菌がプラスミドを取り込んだ。 続いてアンビシリン 有 状送信と発色基質(無色の物質でラクターゼが作用すると青色の物質に変化する)を含んだ寒天培地上 で一晩培養すると、図5に示すように青色と白色のコロニーの形成が観察された。なお、コロ ニーとは1個の大腸菌が分裂・増殖して形成された集落のことを意味する。また、実験に用い た大腸菌は Amp" 遺伝子と lacZ 遺伝子をもたず、アンビシリンの存在下では死滅するものと はんで 11 立子組み換えに使われる 子のはこびやベクター 抗生物質の 作用をおさえる Amp Tacz 図1 ラクトースを分解 酵素(A)による 遺伝子X

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English Senior High

文章を読み200字以内の日本語で要約して欲しいです

Ⅰ 以下の英語の文章を200字以内の日本語で要約しなさい。 ad a We often forget that an important part of "scientific" knowledge was built on the study of alchemy and other magical practices Alchemists were interested in changing certain metals into more valuable ones For example they tried to change lead into gold, However, they also wanted to produce medicines (that would allow people to live forever or cure any disease. The philosopher's stone is known to us today from the Harry Potter series of To novels and movies This magical stone was believed to have enormous powers and make you capable of doing and knowing pretty much everything. 可能にする *John Dee was an alchemist (who was particularly interested in the problem <of foretelling the future from the positions of the stars and other planets He was also an expert in ordinary mathematics and navigation, One of his most * ふつう fell important projects involyed, research (on a universal language (for 巻き込む communicating with angels!Dee was extremely successful He made a lot of money/had (extremely high status (in universities and government, and owned one of the best libraries in Europe/much of it dedicated to magic. 捧げる However towards the end of the sixteenth century/ideas about magic were changing. Many Christians in England were unhappy(that people were still キリスト教入 communicating with the spirit world which was one of the goals of sixteenth century magicians, As you know Japanese people welcome the spirits of ancestors into the house during the Bon festival European Christians were not happy (about that kind of thing, and they complained (about similar European festivals like Halloween) At the same time, many Christians were afraid that alchemists might be trying to steal God's power. As a result, there was a powerful movement to shut down magic once and for all. 禁止する ALE V n You may be familiar with the Japanese manga and anime series *Fullmetal 45% a Alchemist./The story takes place (in a fictional world in which alchemy continues to function as a normal part of scientific knowledge For example> the heroes (of the series are searching for the philosopher's stone,/and alchemists carry out important work(on behalf of the government/If our 利益 modern world had developed (in the same way as the world of Fullmetal Alchemist people like John Dee would probably have continued to do well. In fact, he lost his jobs and money and died in poverty. 1

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Biology Senior High

PCR法 問1がまずまったくわかりません。 どこからこの答えを導いていますか?

ア 19. PCR 法 ①6分 次の文章を読み, 下の問いに答えよ。 PCR 法で DNA を増幅させる場合, 鋳型となるDNA 断片, ァ2種類のプライマー,イ DNAポリメラ ーゼとヌクレオチドを反応させる。 PCR 法では, 約 ウ℃で2本鎖DNAを1本鎖に解離させ,約 エ°Cでプライマーを結合させ,約オ℃で新生鎖を合成させる。 これらのステップを繰り返す ことで, プライマーにはさまれた領域の DNA を指数関数的に増幅させることができる。 問1 下線部アについて,下図のDNA を鋳型として PCR法を行う場合に用いるプライマーとして適当 なものを下の①~⑧のうちから二つ選べ。 なお、図や選択肢中の5′や3′ はそれぞれ5'末端と3 末端を意味するものとする。 5' AACTAGTCGA 3' TTGATCAGCT ① 5′ AACTAGTCGA 3' ④ 5′ GAAGAATTAG 3' ⑦ 5′ CTAATTCTTC 3′ 増幅させたい領域 25' TTGATCAGCT 3' ⑤ 5′ AGCTGATCAA 3′ ⑧ 5′ GATTAAGAAG 3′ CTTCTTAATC 3' GAAGAATTAG 5' ② 高温条件下で変性しにくい。 ③ プライマーから両方向に向かって鎖を伸長させられる。 ④ DNAの合成速度が大きい。 ③ 5′ CTTCTTAATC 3′ 65' TCGACTAGTT 3' OOTDATODOT 問2 下線部イについて, PCR 法で用いるDNAポリメラーゼが備えている必要のある性質についての 記述として最も適当なものを、次の①~④のうちから一つ選べ。 ① DNA分子の特定の領域のみを複製する。 Ate II 遺伝情報の発現 23

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English Senior High

答えを教えてください。

D) LANGUAGE PRACTICE Should and have to Suggestion What should I do? You should try yoga. You shouldn't eat so much fast food! Should I drink more water? Yes, you should. Complete the sentences with should, shouldn't, have/has to, or don't/doesn't have to. 1. To be healthy, we 2. I have a car, so I 3. You 4. My sister is really busy. She should sleep seven or eight hours every night. walk to work. But I still walk for exercise. drink so much soda every day. It's bad for you. 3. You should get some fresh air 4. You shouldn't exercise Grammar Reference page 130 Obligation What do I have to do? You have to read these books. You don't have to do anything! Do I have to pay for the class? No, you don't. 2 Match the information to make complete sentences. 1. We have to pay the fees_e 2. Should we warm up 5. You don't have to go on a diet 6. How much do we have to pay I think people should exercise every day. write ten essays before the semester ends. Unreduced 1. He has to eat first. 2. I have to leave early. 3. She has to go now. 4. They have to pay. a. before we exercise? b. to lose weight. c. after a big meal. d. because you've been indoors all day. e. before we take the class. f. to join the gym? 3 PAIR WORK. What should people do to have a healthy lifestyle? List five things. Then discuss your ideas with your partner. ONLINE PRACTICE Every day? That's too much! PRONUNCIATION-Reduction of has to and have to 1 Listen. Notice the reduced sounds of has to and have to. Reduced He hasta eat first. I hafta leave early. She hasta go now. They hafta pay.

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