Grade

Type of questions

Biology Senior High

PCR法 問1がまずまったくわかりません。 どこからこの答えを導いていますか?

ア 19. PCR 法 ①6分 次の文章を読み, 下の問いに答えよ。 PCR 法で DNA を増幅させる場合, 鋳型となるDNA 断片, ァ2種類のプライマー,イ DNAポリメラ ーゼとヌクレオチドを反応させる。 PCR 法では, 約 ウ℃で2本鎖DNAを1本鎖に解離させ,約 エ°Cでプライマーを結合させ,約オ℃で新生鎖を合成させる。 これらのステップを繰り返す ことで, プライマーにはさまれた領域の DNA を指数関数的に増幅させることができる。 問1 下線部アについて,下図のDNA を鋳型として PCR法を行う場合に用いるプライマーとして適当 なものを下の①~⑧のうちから二つ選べ。 なお、図や選択肢中の5′や3′ はそれぞれ5'末端と3 末端を意味するものとする。 5' AACTAGTCGA 3' TTGATCAGCT ① 5′ AACTAGTCGA 3' ④ 5′ GAAGAATTAG 3' ⑦ 5′ CTAATTCTTC 3′ 増幅させたい領域 25' TTGATCAGCT 3' ⑤ 5′ AGCTGATCAA 3′ ⑧ 5′ GATTAAGAAG 3′ CTTCTTAATC 3' GAAGAATTAG 5' ② 高温条件下で変性しにくい。 ③ プライマーから両方向に向かって鎖を伸長させられる。 ④ DNAの合成速度が大きい。 ③ 5′ CTTCTTAATC 3′ 65' TCGACTAGTT 3' OOTDATODOT 問2 下線部イについて, PCR 法で用いるDNAポリメラーゼが備えている必要のある性質についての 記述として最も適当なものを、次の①~④のうちから一つ選べ。 ① DNA分子の特定の領域のみを複製する。 Ate II 遺伝情報の発現 23

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TOEIC・English Undergraduate

自分の回答があっているか不安なので、4第答えて頂きたいです。 自分の回答はこちらには見にくくなってしまうため書いていません。

Working with words 1 Complete the review of a hotel. Use the answers to complete the puzzle and find the European city where the hotel is located. HOTEL REVIEWS This first-class hotel and conference centre welcomes guests from all over the world. Its 1 facilities are second to none. There are 300 en-suite rooms and five apartment suites. For business guests, it has ten meetings rooms, two of which are big enough to be used as halls. 2 The hotel can also organize events such as guided 3 around the town centre for conference 4 and hotel guests who enjoy a bit of 5 For food-lovers, the four-star restaurant serves regional 6 every evening. All in all, this is a top-class for business and for pleasure. 7 6 1 A fa 0 il i t 1 e S 2 Replace the words in italics (1-8) with the phrases from the list. Add a pronoun if necessary. you hotel? look around meet up with show someone around 3 freshen up eat out pick someone up check in drop someone off Pedro It's difficult to park here. Can I stop and leave drop you off in front of the 1 Sabrina Sure, I'll register 2 and then I'd like to have a wash, and change my clothes 3 Pedro If you like, tonight I can give you a tour of 4 the old city. We could by eat in a restaurant 5 the port. Sabrina That sounds great! I'd rather walk about and See 6 the city than stay in my hotel room. Pedro I'll collect you 7 8 8.30 p.m. We'll see Alberto and Maite in the main square. at Business communication 1 Put the words in the correct order to make expressions. 1 meet person / it's / nice / to / you / in It's nice to meet you in person 2 have / did / finding / you/ any / trouble / us? 3 worry / signing / don't / about / in 4 through / programme / I'll / run / today's 5 this my / come / way / to / office 6 need building / you'll / this / enter / badge/ the / to 7 reception / sure / make / in / at / you / sign 2 Raymond Roberts has an appointment with Janet Rose. He has just arrived at HBG premises. Complete their conversation with the phrases from the list. let me take your bag can I get you a drink Welcome to HBG publishing I thought you could catch up again how was your journey You'll need this Make sure you 1 Raymond Good morning, I'm here to see Janet Rose. Janet Hello, I'm Janet. ¹ Welcome to HBG publishing. Raymond It's nice to meet you in person. Janet Likewise. So, 2 Raymond Well, there were traffic jams on the motorway and I got a little bit lost in the industrial park. Janet Don't worry. That happens to everyone. Anyway, 3 - I'll store it in my office. Raymond I'll hang on to it if you don't mind. It's got all my stuff in it. Janet Well, if you change your mind just tell me. And 4 Raymond Thanks. I'll have a cup of tea, please. Janet Sure, I'll just get that for you in a second. First of all, I'll run through the schedule. 5 start by meeting Karen Rankin this morning and then we'll 6 at lunchtime. Raymond OK. And will I see Malcolm Briscoe? Janet Yes, in fact he's joining us for lunch. One other thing. security pass. at all times. 7 It's your 8 wear it

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