Grade

Type of questions

Biology Senior High

生物のプラスミドの実験の問題です (4)(5)の解き方を教えていただきたいです🙇‍♀️

(1) プラスミドAの転写調節についての、以下の問い①~③に答えよ。ヒーロー プロモーターに結合するものは何か。出 lat② オペレーターに結合するものは何か。 YV 5 ③ X-gal は, β-ガラクトシダーゼで分解されるが、 同じくこの酵素の基質であるラクトース とは違い, β-ガラクトシダーゼの発現を誘導しない。 β-ガラクトシダーゼの発現を誘 導させるために培地に加えたIPTG は、 何と結合するか 16 12 のである 表 2 に示す結果で,以下の(a)~(d) そ れぞれのコロニーに含まれる大腸菌はどれか。 下の解答群の1)~4) の中から, あてはまるもの をすべて選べ。 (2) 制限酵素 H は, DNA 中のある特定の6個の塩基配列を認識して, その部分を切断する。 業中 本鎖DNA に A, G, T, C が偏 (かたよ) りなく分布していると仮定すると, 制限酵素 Hが認 実識する塩基配列は何塩基につき1回出現すると推定されるか t カナマイシ (3)表 2 は操作8で出現したコロニー数をまとめたも 6024 4 表2 大腸菌のコロニー数 大腸菌の 計数に使用 希釈菌液 した培地 →4046 出現した コロニー数 *** (a 培地で培養して生じたコロニー 培地 2 110 (b) 培地2で培養して生じたコロニー TasV & tu 白色 25 希釈菌液 1 培地3 12d 青色 95 培地 4 4 160 希釈菌液3 培地 1 50 (c) 培地で培養して生じた白色のコロニー (d)培地3で培養して生じた青色のコロニー [解答群] モニ ***各希釈菌液の 0.1mL を寒天培地に プラスミドを取りこまなかった大腸菌 ② lacZに外来遺伝子が組みこまれていな プラスミドAを取りこんだ大腸菌 滴下して塗り拡げた。 ③ lacZに外来遺伝子が組みこまれたプラスミドAを取りこんだ大腸菌 ④ lacZにカナマイシン分解酵素の遺伝子の全部が組みこまれたプラスミドAを取りこみ、 組みこまれた遺伝子が発現した大腸菌 (4) 表2に示す結果をもとに, 操作(4)でDNAを混合した後の大腸菌液1mL中の, 以下の(a)~ (c)に示す大腸菌の数を求めよ。 計算結果はa× 10°の形式で表し, b は整数で答えよ。 ただし, 1つのコロニーは1個の大腸菌細胞に由来するものとする。 ① すべての大腸菌 ② lacZに外来遺伝子が組みこまれたプラスミドAを取りこんだ大腸菌 カナマイシン分解酵素の遺伝子の全部が組みこまれたプラスミドAを取りこみ, その遺伝子が発現した大腸菌 (5) 操作(4)でDNAを混合した後の大腸菌の菌液1mLに含まれるすべての大腸菌の中で, プラ スミドAに組みこまれたカナマイシン遺伝子が発現した大腸菌の割合を求めよ。 計算結果は, 百分率(パーセント) で答えよ。 021 2 001 001 [中央大〕 0 0 Day WORK - ROV

Waiting for Answers Answers: 0
English Senior High

(6)の模範解答が⭕️なのですが、問題文にはwhen Nightingale was young とあり、文中の黄色マーク🟡で引いた該当する文は、彼女が30歳になった時のことを言っているのから❌ではないのですか?教えてください🙏😭

次の英文を読んで、(1)~00までの文がその内容とあっていれば〇をそうでなければ×を解答 用紙に記入しなさい。 Florence Nightingale Florence Nightingale was born on May 12, 1920, into a wealthy family in England, and received the most luxurious education from an early age, learning not only foreign languages like French, Greek, and Italian, farmers she visited for charity work, she gradually began to think that she wanted to work in a job that but also mathematics, astronomy, psychology, and literature. However, after seeing the lives of poor served people. When she turned 30, she decided to become a nurse and started working at a hospital in London. Nightingale, who eventually became a director of a women's hospital, began to advocate the need for nurses with specialized training. At that time, nurses had a low status and were considered nothing more than servants who cared for the sick. A major turning point occurred in 1854. War began in Crimea*, present-day Ukraine, and Nightingale was sent there with 24 Catholic sisters and 14 nurses. Nightingale's efforts improved the hospital environment during the war. The Nightingale School of Nursing was established with the Nightingale Fund created during the war. Although Henri Dunant, a founding member of the International Committee of the Red Cross, highly praised her work, Nightingale was not involved in the International Committee of the Red Cross. This was because she believed that aid activities based on self-sacrifice by participants would not last long. Her famous quote, "Devotion without sacrifice is true service," expresses this well. It is said that this was due to the idea that "we rely on the spirit of service of our members, but without financial support, we are powerless." Nightingale only served wounded soldiers as a nurse for only two years during the Crimean War*, and became famous for her symbolic image of dedication and for her use of statistics to reform health care. The statistical methods she used at this time were highly praised, and she was considered a pioneer of statistics in England. Nightingale suffered from poor health from a young age, and is said to have spent most of her time in bed after returning from Crimea. Nightingale passed away peacefully at the age of 90 at her home in London on August 13, 1910. advocate* 主張する Crimea* クリミア半島 Crimean War* クリミア戦争 (1) Florence Nightingale was born in a wealthy family and she learned many foreign languages. (2) Nightingale wanted to be a nurse when she was small. P (3) It was when she was 30 years old that Nightingale wanted to be a nurse and started working at a hospital. (4) Nightingale's work in Crimea improved the environment of the hospital there. (5) Nightingale did great work to found the International Committee of the Red Cross. (6) When Nightingale was young, nurses were thought to be like servants. (7) Nightingale's famous words, "Devotion without sacrifice is true service," means self-sacrifice of the participants is always necessary rather than financial support. (8) Nightingale was not blessed with good health since young and spent much of her time in bed. (9) Nightingale is considered a pioneer of statistics in the world as she used statistics to reform health care. (10) Nightingale worked as a nurse all her life.

Resolved Answers: 1
English Senior High

英語の問が分からないので誰か解ける人解説込みでお願いします

CHAPTER 4 関連英文 "ninge som ow lit andarwood, dodal Passage 1: Australian Woman Who Died after Battling Rare Cancer Penned Inspirational Viral Letter: Each Day is a Gift' ・戦い戦闘 珍しい希少 brow adi b A 27-year-old Australian woman who lost her battle with a rare form of cancer asked her family to brovndaimuw loline how t share the last letter she wrote on her deathbed, 臨終、臨終の床 bed ada li vorf beslás ban obished alloft t Duralin 08 od nesto lana yad al Holly Butcher's last words soon went viral on Facebook after being posted on January 3, one day I rugged one dado dae Prow of an before she passed away, with more than 131,000 people sharing it on the social network. Niggad evil of bedbow Jaritannig gid sysd tabibl 在住居住者 ソーシャル・ネットワーク aid og H Holly, who resided in Grafton in New South Wales, Australia, began her lengthy note by saying that vidiberon and boa she planned to write "a bit of life advice." 実現する 変怪、奇怪な 死亡率 aude doos bad ead.. sailinil orie “It's a strange thing to realize and accept your mortality at 26 years young. It's just one of those things you ignore," she started. “The days tick by and you just expect they will keep on coming; until 20nd ablo ed ad ayawin lliw dad.blow on the unexpected happens." 予想外、予期せぬ 思いがけない 傷つきやすい静 予測不能不透明 Continuing, she wrote, “That's the thing about life. It is fragile, precious and unpredictable and each day is a gift, not a given right. I'm 27 now. I don't want to go. I love my life. I am happy. I owe that to my loved ones. But the control is out of my hands." i delo at guiwolle ads to doid W (B belustai tog Holly then encouraged her family and friends to stop whining “about ridiculous things. " 勇気づけられた 軽微な問題 あほらしい 提案された ばかばかしい 認める承認 “Be grateful for your minor issue and get over it," she suggested. “It's okay to acknowledge that something is annoying but try not to carry on about it and negatively affect other people's days." thegriot yllauen aw ob ネガティブに否定的H うるさ Holly also advised that people don't "obsess” over their bodies and what they eat.dla sV アドバイス 誓うる 助言 とりつくろう 取り憑 audul art ni sunitaoo lw asvil lieb m “I swear you will not be thinking of those things when it is your turn to go," she wrote. “It is all SO insignificant when you look at life as a whole.” 軽微、取るに足りない 微々たるもの After advising her family and friends to closed her letter by encouraging them to aged liw tedw toibong avawl se their money “on experiences” instead of presents, Holly use their merit huuore algoog art nodaum の代わりに ではなく give back. yasaesoonnu yilshom riodigandinemal 善行 ぜんこう “Oh and one last thing, if you can, do a good deed for humanity (and myself) and start regularly amaldory juoda daum col pai donating blood," she wrote. “It will make you feel good with the added bonus of saving lives.” 寄附 寄付 人命救助 命を救う

Resolved Answers: 1