Grade

Type of questions

Biology Senior High

生物のプラスミドの実験の問題です (4)(5)の解き方を教えていただきたいです🙇‍♀️

(1) プラスミドAの転写調節についての、以下の問い①~③に答えよ。ヒーロー プロモーターに結合するものは何か。出 lat② オペレーターに結合するものは何か。 YV 5 ③ X-gal は, β-ガラクトシダーゼで分解されるが、 同じくこの酵素の基質であるラクトース とは違い, β-ガラクトシダーゼの発現を誘導しない。 β-ガラクトシダーゼの発現を誘 導させるために培地に加えたIPTG は、 何と結合するか 16 12 のである 表 2 に示す結果で,以下の(a)~(d) そ れぞれのコロニーに含まれる大腸菌はどれか。 下の解答群の1)~4) の中から, あてはまるもの をすべて選べ。 (2) 制限酵素 H は, DNA 中のある特定の6個の塩基配列を認識して, その部分を切断する。 業中 本鎖DNA に A, G, T, C が偏 (かたよ) りなく分布していると仮定すると, 制限酵素 Hが認 実識する塩基配列は何塩基につき1回出現すると推定されるか t カナマイシ (3)表 2 は操作8で出現したコロニー数をまとめたも 6024 4 表2 大腸菌のコロニー数 大腸菌の 計数に使用 希釈菌液 した培地 →4046 出現した コロニー数 *** (a 培地で培養して生じたコロニー 培地 2 110 (b) 培地2で培養して生じたコロニー TasV & tu 白色 25 希釈菌液 1 培地3 12d 青色 95 培地 4 4 160 希釈菌液3 培地 1 50 (c) 培地で培養して生じた白色のコロニー (d)培地3で培養して生じた青色のコロニー [解答群] モニ ***各希釈菌液の 0.1mL を寒天培地に プラスミドを取りこまなかった大腸菌 ② lacZに外来遺伝子が組みこまれていな プラスミドAを取りこんだ大腸菌 滴下して塗り拡げた。 ③ lacZに外来遺伝子が組みこまれたプラスミドAを取りこんだ大腸菌 ④ lacZにカナマイシン分解酵素の遺伝子の全部が組みこまれたプラスミドAを取りこみ、 組みこまれた遺伝子が発現した大腸菌 (4) 表2に示す結果をもとに, 操作(4)でDNAを混合した後の大腸菌液1mL中の, 以下の(a)~ (c)に示す大腸菌の数を求めよ。 計算結果はa× 10°の形式で表し, b は整数で答えよ。 ただし, 1つのコロニーは1個の大腸菌細胞に由来するものとする。 ① すべての大腸菌 ② lacZに外来遺伝子が組みこまれたプラスミドAを取りこんだ大腸菌 カナマイシン分解酵素の遺伝子の全部が組みこまれたプラスミドAを取りこみ, その遺伝子が発現した大腸菌 (5) 操作(4)でDNAを混合した後の大腸菌の菌液1mLに含まれるすべての大腸菌の中で, プラ スミドAに組みこまれたカナマイシン遺伝子が発現した大腸菌の割合を求めよ。 計算結果は, 百分率(パーセント) で答えよ。 021 2 001 001 [中央大〕 0 0 Day WORK - ROV

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English Senior High

教えてほしいです

3 次の英文を読み, 空所に入れるのに最も適切なものを, それぞれ下の①~④のうちから一つずつ選びなさい。 Cigarette smoking has negative effects not only on the body of the person who smokes but also on the body of someone who regularly breathes in second-hand smoke. Some of the most obvious effects are high blood pressure, sleep disorders, heart failure, and lung cancer. Despite such harmful consequences, ( 10 ). It has been reported that smoking creates feelings of pleasure, reduces tension, and promotes close relationships. Many smokers admit that smoking is a bad habit. ( 11 ), they tend to think that the number of cigarettes that they smoke is below the danger level and thus are not worried about the risk. Some even feel that they do not breathe in the smoke and that a cure for cancer will be found before they become ill with the disease. We have to admit that smoking is a habit which is difficult to break ( 12 ) the nicotine found in tobacco goes into the blood and stimulates the brain, making smokers feel pleasant for several minutes. What is more, the smoker usually feels anxious and wants to have another cigarette. ( 13 ) the policies that now ban smoking in most public areas, the stress that people often experience at work and at home can force them to smoke. ( 10 ) ①some people decide to stop smoking millions of people continue to smoke ( 11 ) the price of cigarettes has risen ①smoking is more harmful than drinking Therefore 2 While ③ Of course 4 However (12) 1 because 2 as a result 3 but SO (13) ①Despite 2 Although (3) But 4 Yet

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English Junior High

(1)の答えがD、(3)の答えがウ、(6)の答えがイ なんですけど、なんでそれになるかと、5⃣で3~5程の段落で分けるとしたらどこになるのか、またその段落はそれぞれどんな題名になるのかを教えて欲しいですm(_ _)m

学院) E J 5 次の英文を読んで、あとの問いに答えなさい。 < 星野改〉 High schools in Minnesota have a problem Many students are late to school. They are often tired. Some students fall asleep in class. They often get sick, too. The local governments take that as a problem. They make a small change.) It helps a school day a little later/ A lot! What do they do? They start the D- This small change makes a big difference. Why? Most teens are very tired early in the morning. They usually don't go to bed until after midnight In the morning, they wake up between 6:00 and 6:30 for school. So they don't get enough sleep! They are still tired early in the morning. But after the change/ teens are more awake and later start time is better for a teen's body clock I be ready/class/to/in). A be in ready class Everyone has a body clock. An adult's body clock works like this: most adults get tired between 9:00 and 11:00 at night. They usually go to bed before midnight. Adults can get up early in the morning. It's not a problem. But a teen's body clock is different. Teens don't get tired at midnight. They usually stay up later. But early in the morning, they need more sleep. B FO C The schools in Minnesota pay attention to the teen's body clock. They change the start of the school day from 7:20 a.m. to 8:40 a.m., 80 minutes later. Other schools in the United States change their start times too. Some schools change the time by only 30 minutes, but they still get good results. ( 2 ), the results are amazing! ③(Many) students are on time. Morning classes are easier to teach. Students are getting ①(good) grades. Students have fewer illnesses, so they are ⑤(little) absent. In Minnesota, there is another important effect: fewer students drop out of school or change schools. D Today, more and more high schools are starting later. Most schools can't start two hours later. But they can change the start time a little. A small change can make a very big difference! Just ask the students. Minnesota ミネソタ (アメリカ合衆国中央北部の州) (1) 次の英文を入れるのに最も適する位置を, 本文中のA~Dから1つ選びなさい。 Now the students are happier, and also the parents and teachers too. D(A) (2)下線部①の ( )内の語を並べかえて正しい英文を作るとき, 3番目にくるものをア~エから1つ選びなさい。 ア be イ ready ウ class I to (3) 20)( )に適するものを, ア~エから1つ選びなさい。 7 On the other hand (4) 3~50)( 1 However ウ In fact I Even so )内の語をそれぞれ比較級にかえなさい。 (3) much (5)本文の内容にあうものを,ア~エから1つ選びなさい。 more (T!) better ⑤⑤ fewer Less High school students in Minnesota are always taking classes seriously. Most teens usually go to bed as early as possible. Most adults don't stay up late because of their body clocks. I Some schools changed the school time but their students did not have good grades. 本文のタイトルとして最も適するものを,ア~エから1つ選びなさい。 7 Fewer Illnesses 1 Late Start Body Clock I Good Sleep イ (土)

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English Junior High

(1)の答えがchoosing、(2)の答えがウ、(5)の答えがアで、それぞれなんでその答えになるのかと、 5⃣の本文を上から4行、4行、5行、4行、3行、2行で分けた時それぞれに題名を付けるとしたらどうなりますか? 教えて欲しいですm(_ _)m

5 次の英文を読んで, あとの問いに答えなさい。 <川越東改> Origami is the Japanese art of folding paper. To do origami, the artist starts with a square piece of paper. Some people like to use special origami paper that is two colors. The front of the paper is one color, and the back of the paper is another color. Other people like to use origami paper that has patterns on it. After ①(choose) your paper, you can find many instructions for folding the paper into different things. Many people enjoy (make) origami flowers, animals, or things ( 3 ). For all of these things, there are a few kinds of folds that you need to use. For example, sometimes you need to fold the paper in half. Sometimes, the paper must be folded from corner to corner. If you follow the directions carefully, you can create a beautiful paper flower or animal. However, origami is more than folding paper. First, origami is an important part of Japanese life. For example, nature is important in Japan. In Japan, people care about the seasons, weather, water, or other things in nature. Origami is also a part of nature. That is why the most popular origami shapes are things like animals. Birds, fish, flowers, and stars are all popular shapes. It is a quiet activity, and can calm the mind and body. People who do origami like the activity as much as the art. They like it because origami demands a lot of attention. When people think hard about creating something, they forget about their problems. This allows them ④ to calm down. ⑤ Origami is also good for teaching children. They also learn to work carefully. Also, origami has squares and triangles. These shapes are important in all kinds of learning. Origami helps children to learn about these shapes. Maybe you can try to do origami yourself. You only need some paper and a book of instructions. You can find instructions for many origami shapes on the Internet. instruction direction (1)①,②の( )内の語を適する形にかえなさい。 (2) 30( に適するものを, ア~エから1つ選びなさい。 (3) (4) 7 to paint with 1 to talk with 1 (2) making. to play with I to help with (イ) ④に適するものを,ア~エから1つ選びなさい。 Origami is also easy to learn. 1 Origami is also good for your imagination. Origami is also difficult to learn. I Origami is also good for your mind. ⑤にはA~Cの文が入ります。 自然な流れの文章になる配列を, ア~エから1つ選びなさい。 A Children must follow these steps exactly. B First, origami has many steps. C This way, children can learn to follow instructions. ア A-B-C イ A-C-B B-A-C I B-C-A (5)本文の内容にあうものを, ア~エから1つ選びなさい。 If you follow some instructions for paper folds, you can enjoy many different origami shapes. Learning origami gives us a good chance to help animals on the earth. You may feel tired if you try hard to do origami carefully. I The most important thing for children's education is origami. (土)

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TOEIC・English Undergraduate

至急お願いします!

次の1~5の日本文の意味を表すように、 語を補いなさい。 )内の語(句)を使って、下線部分に適切な英 1. 彼女は給油するためにガソリンスタンドに立ち寄った。 (car, fill up) 《英語》She stopped 2. 私は日本に私の服を送るための箱を探している。 (clothes, box, Japan, send) 《英語》I am looking for 3.彼はファイナンシャルプランナーになるために勉強している。 (become, financial, study) 《英語》 He is 4. 彼女は子供に料理を教えることのできる場所を借ります。 (cooking, kids, place, teach) 《英語》 She rents 5. キャシーは彼に仕事を引き継いでもらいたがっていた。 (business, take over) 《英語》 Cathy wanted at a gas station. planner. Ⅱ 次の英文を読んで、 下記のペアワークやグループワークに取り組みましょう。 ◎ CD 70 DL 70 A good work-life balance enables us to divide our energy between our home and work priorities. It also enables us to reduce stress and anxiety both at work and at home. In an effort to strike an optimum work-life balance, I struggle to find anything like a balance between work and doing something for myself at all. I want to travel to places in Asia to diversify my life. I hope to stay physically and mentally fit. I hope that my life will not always be as busy as it is right now. Notes 1. enable O to do 「○が…することを可能にする」 2. divide ○ ○ を分ける」 3. priority 「優先事項」 4. reduce 「○ を減らす」 5. optimum 「最適な」 6. struggle to do 「・・・ しようと努力する」 7. at all 「とにかく」 8. diversify ○ ○ に厚みを持たせる」 9. stay C 「Cのままでいる」 Pair/Group Work ペアまたはグループになって質問をしたり、答えたりしましょう。

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