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J0) 12.4 Write B's questions. Use: Cost get to work go go to bed late happen have a nice time Stay win 1 A: We went to Chicago last month. B: Where didyou stay 5 A: We came home by taxi. B: How much A: With some friends. A: Twenty dollars. 2 A:Iwas late for the meeting. B: What time 6 A: I'm tired this morning. B: A: 9:30. A: No, but I didn't sleep very well. 3 A:Iplayed tennis this afternoon. 7 A: We went to the beach yesterday. B: B: A: No, I lost. A: Yes, it was great. 4 A:Ihad a nice vacation. B: Good. Where A: To the mountains. 8 A: The window is broken. B: How A: I don't know. 12.5 Put the verb in the correct form - affirmative, negative, or question. 1 We went to the movies, but the film wasn't very good. We didn'tenjoy. it. (enjoy) 2 Tom Some new clothes yesterday - two shirts, a jacket, and a sweater. (buy) yesterday?" "No, it was a nice day" (rain) long at the party. (stay) 3 4 We were tired, so we a window. (open) time" (have) that?" (do) It was very warm in the room, so l 6 "Did you call Chris this morning"" "No,1 7 "Icut my hand this morning," "How 8“Why weren't you at the meetingyesterday?". "1 about it" (know) 25

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RELATIVE CLAUSES 1)That's the sheep ……I saw yesterday. 2)He bought the 5.- Mary was the girl had a bath. 3) Paul is the boy 4)Iwashed my car souvenirs he … calculator I'm … was very dirty. a)who wanted. using. a) whose a) which b) who c)whom a) who b)that c)whose a)whom b)which c) whose b)whose b) who c)that c) which a 7.- Anne hasa 9.-The book we 10.-Ten o'clock is the time I go 6.- The birthday … 8.- Julio is the er. person with he is working there. we ate was delicious. school bag she read in bed is carries her books. fantastic. home. a)which b)who c)where a)who b)where c)that a)whom b) which c) who a)which b)whose c)who a) whose b) when c) who 0S. 15.-Those are the 13.-Tobby was the dog died last night. 11. - She opened the 12.-I visited Notre 14.-He had the box.. she received. accident he grapes We bought Dame.. is in Paris. was skiing. a) that b) where c) who a) who b) which c)where a)which b)when c)whom a)when b)who c)where a) where b that c)when ect 19.-The girl.. 20.- The armchair 16.- They are the me children . parents 17.-My dad did the 18.-The man was asked the question …… he sat down laundry was in the basket. in charge of the are Dutch.oid factory wasn't was my sister. was green. Roger. a)who b)whose c)where a)where b)whom c)who a) which Women a)who a)where to? b)who b)who of stars c)when b) whom The one or Tivse c)whom c)whose 21.-He 22.-Eric is a French 23.-An architect is 24.-The day .. we 25.-The house Wore a costume man ..wife is somebody. meet was raining. we lived was was strange. Spanish. designs buildings. enormous. a) where b)which c)who a)which b)who c)whose a)who b)when c)whom a)whom b)when c) which a)which b)when c)where 29.- Lola called a 30.-The kid played 26.-The film I saw 27.-Sushi is food 28.He played friend was in video games..…… was frightenng. …… Japanese eat. football he met Amsterdam. belonged to dad. his friends. a)that a)which a)which a)whom a)whom b)who b)where b)who b)who b) who c)where c)who c)when c)where c)which

2),(4),(5),(6),(7)は非限定用法になるのですが、限定用法と非限定用法にするときの区別がいまいちわかりません。 先行詞が名前や年とかの時は非限定用法ということはわかります！なにか簡単に見分ける方法はありますか? 英語表現

3 下線部を先行詞とする関係代名詞を用いて, 2つの文を1文にしなさい。A CD 1. These are the shoes. I paid $69.99 for them. I thought it safer. 2. He told me to stay home. 3. The medicine cured the disease. I had suffered from it for a long time. That made me happy. It is written in easy English. 4. My brother passed the entrance exam. 5, Meg recommended this book. 6. Do you remember John Smith? 7. The teacher said the questions were easy. He sent us a letter before. C It turned out to be false.

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ー 比較級(~er)+than =「…よりも~」 次の に,( )内の語を適する形にかえて書きなさい。 ヒント 0 My dog is .. b139eh. .elder Longer. neweh. than yours.(big) good「よい,じょうず な」,well「じょうずに」 の比較級は 2 Yuki is than Keiko. (old) の Canada is than Japan.(large) hetter です。 の Your bag is than mine.( new) ③ Iget up __eahlier. than my sister. (early) 6 Mike can swim faster than Ken. ( fast) の Iam _ buyet. @ Koji's computer is gaoder 0 This question is eatyet の I am than my sister. (busy) than mine.(good) than that one.(easy) ① Emi speaks English betten than Aki. (well) 日本文に合う英文になるように, に適する語を書きなさい。 0 わたしのねこはあなたのよりも小さい。 My cat is SMallen than yours. 2 トムは彼のお父さんよりも背が高い。 Tom is talle than his father. 由美は恵子よりもじょうずにピアノをひけます。 Keiko. Yumi can play the piano の この机はあの机よりも古い。 that one. This desk is 英語の重点ドリル2

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次の に,()内の語を適する形にかえて書きなさい。 of the five.(big) ヒント 0 My dog is the bigest. y0shgest in her family. (young) mountain in Japan. (high) good「よい、 ょうずな」、we 「じょうずに」の Yuki is the ③ This is the Kighest. 上級は of all.(new) best ④ This computer is the 1newest です。 O Yumi gets up the _eanlyest in my family. ( early ) fastest in his class. (fast) of the four.(good) 6 Mike swims the の Koji's bag is the ③ Tom is the _ tallezt. in his family.(tail ) O This question is the of all.(easy) ③ Emi speaks English the in her school.(well) 2 日本文に合う英文になるように, に適する語を書きなさい。 0 恵子は5人の中でいちばん年上です。 Keiko is of the five. 2 わたしの母は家族の中でいちばん忙しい。 My mother is in my family. 3 このバッグは4つの中でいちばん小さい。 This bag is the fe 由美はクラスの中でいちばんじょうずにテニスをします。 Yumi plays tennis h のそ上 1*11 n4:

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課題 D-3 下の図を参考に次の文章の空欄を埋めよ 細胞膜はタンパク質を通さない。そこでタンパク質を分泌するためには(① )と呼ばれ る特別な装置が必要である。分泌されるタンパク質には( 12 )に( 13 )ペプチドがつ いた形で翻訳される。これは分必される際に膜上の( 0 )によって切断され、( 5 )タ ンパク質となる。 PET226は( 16 )という®を付加できるようになっており、タグは(① ) に付加できるようになっている。 大腸菌で大量にタンパク質を作らせると、( 1® )を形成することがある。これはタンパク 質の正しい折りたたみ( 19 )が追いつかないことが主な原因である。そこで、生物が本 来持っている、折りたたみを補助するタンパク質群である( 20 )を増強した大腸菌を用 いることで、回避できる可能性がある。 PET226 マルチクリーニングサイト周辺 T7 promoter primer #69348-3 Bgl II T7 promoter lac operator Xbal rbs BSPMI pelB leader MscI Ncol Ndel BamHI EcoRI Sacl MetLysTyrLeuLeuProThrAlaAlaAlaGlyLeuLouLeuLeuAlaAlaGInProAlaMetAlqMetAsp1leGlyIleAsnSerAspProAsnSerSerSer Sall Hind II Eag」 Not」 Aval Xhol His-Tag signal peptidase Bpu11021 ValAspLysLeuAlaAlaAlaLeuGluHIsHisHisHIsHIsHisEnd T7 terminator T7 terminator primer #69337-3

生物の問題の(4)がわかりません！答えは2枚目の写真になるんですが4分の1はどこから出てきたのでしょうか？

場合,生じる DNA断片の平均の塩基対数はいくつになると考えられるか。次の(ア)~(カ)から選べ。 ま た。 DNA材料2:プラスミドDNA DNA材料1:タンパク質Xの遺伝子を含むDNA AATTCCC CCCTTAA 【実験) 0目的の DNA をプラスミド DNA に挿入するため, まず,プラスミド DNA を行はさみ のような役目をする酵素 2 次に1のりのような役目をする酵素を,目的のDNA と, 切断したプラスミド DNA の入った落 液に入れて反応させ, 環状の DNA をつくった。 ③ この環状 DNAを大腸菌に取りこませた後,この DNA を含む大腸菌だけを増殖させた。 ④ 増殖させた大腸菌から, この現環状 DNA を大量に調製した。 (1) プラスミドとは一般にどのようなものか説明せよ。 で切断した。 1 の S (2) 下線部ア)の酵素の総称, 下線部(イ)の酵素名を答えよ。 (ア) ](イ)[ り (3) 実験の酵素口1に適する酵素を, 下記の(a)~(c)から選び, 記号で答えよ。ただし、 破線は 酵素の DNA 鎖の切りかたを示す。また,各酵素は上のプラスミド DNA の図で塩基配列が有 略されている部分は切断しないものとする。 (a) BamHI GGATCC (b) ECORI GAATTC (c) Smal CCCGGG CCTAGG CTTAAG GGGCC (4)(3)の(a) BamHI は6塩基対の配列を認識して切断する。ある DNA を BamHI により切断した 場合,生じる DNA断片の平均の塩基対数はいくつになると考えられるか。次の(ア)~切 使る ただし,切断した DNA の塩基配列は,ランダムであると仮定する。 (ア) 160 (イ) 460 (ウ) 4000 (エ) 40000 (オ) 160000 (カ) 240000 67 | ィ=

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e 4 think re mote dant chool have best We way 90 every have ihe ike Cant Teachers 10 t0 Cah dy we SO I don4 Alsen ナme alane more Jenrning ith tll Cutyat beca we I some quesilas 10 40 語

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It is I of a You I am a , but new create for the . s6uIM Y全首 Leonardo da Vinci If I had レオナルドダヴィンチ uhua ep nopw:pue:m the across ages. They led to the ir My dream is to invent a time machine. long time, ways. one, I would visit areat inventors things stary This is the first t" 04 I have learned from past dreamers ho new. The Wright Br as dreams. flight in 1903. Bef experinents. They 01 again. Sometimes, times, the brothers Leonardo da Vinci. He thought, “I wish I couid flv liu. However, they ne- a bird." He and others studied birds. They watched problem, they lo feathers in the wind. Gradually they learned some of the that they had t S1 secrets of flight. afraid of it. Th

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The stories taught me two . First, we Using our is the key to off. , JRead ST STAGE 1 Ge ロロbロ. sitate [hézatei 次の2つの名言を読』 0Opportunitiee めらう ②) Genius is one our imagination is the key to STarTing off. Secon Using toff 出発する should not hesitate to come up with new and original ide jeueszgdj [Buo STAC 2 Guid- 陸がスピーチで Guice2 (1)偉人たちの (2)偉人たちの to imagine and the courage to act in your personal and publiclives. Goci スピー Thank for listening. nok Messag