学年

質問の種類

生物 高校生

生物の問題の(4)がわかりません!答えは2枚目の写真になるんですが4分の1はどこから出てきたのでしょうか?

場合,生じる DNA断片の平均の塩基対数はいくつになると考えられるか。次の(ア)~(カ)から選べ。 ま た。 DNA材料2:プラスミドDNA DNA材料1:タンパク質Xの遺伝子を含むDNA AATTCCC CCCTTAA 【実験) 0目的の DNA をプラスミド DNA に挿入するため, まず,プラスミド DNA を行はさみ のような役目をする酵素 2 次に1のりのような役目をする酵素を,目的のDNA と, 切断したプラスミド DNA の入った落 液に入れて反応させ, 環状の DNA をつくった。 ③ この環状 DNAを大腸菌に取りこませた後,この DNA を含む大腸菌だけを増殖させた。 ④ 増殖させた大腸菌から, この現環状 DNA を大量に調製した。 (1) プラスミドとは一般にどのようなものか説明せよ。 で切断した。 1 の S (2) 下線部ア)の酵素の総称, 下線部(イ)の酵素名を答えよ。 (ア) ](イ)[ り (3) 実験の酵素口1に適する酵素を, 下記の(a)~(c)から選び, 記号で答えよ。ただし、 破線は 酵素の DNA 鎖の切りかたを示す。また,各酵素は上のプラスミド DNA の図で塩基配列が有 略されている部分は切断しないものとする。 (a) BamHI GGATCC (b) ECORI GAATTC (c) Smal CCCGGG CCTAGG CTTAAG GGGCC (4)(3)の(a) BamHI は6塩基対の配列を認識して切断する。ある DNA を BamHI により切断した 場合,生じる DNA断片の平均の塩基対数はいくつになると考えられるか。次の(ア)~切 使る ただし,切断した DNA の塩基配列は,ランダムであると仮定する。 (ア) 160 (イ) 460 (ウ) 4000 (エ) 40000 (オ) 160000 (カ) 240000 67 | ィ=

未解決 回答数: 1
生物 高校生

下記の問題を教えて欲しいです

B) 欠失 口の部分を欠失させる場合 部位特異的変異導入の実際 5-CGTCGTTTTACAACGTCGTGACITGGGAAAACCCTGGCGTTACCCAAC-3' 3-GCAGCAAAATGTTCGAGCACTGAてCCTTTTGGGACCGCAATGGGTTG-5' * 欠失を行う のより正確なPCR用DNAポリメラーゼを使う 2変異の入ったPCR用プライマーの設計 変異を入れたいところを中心に、前後の広い範囲の 塩基配列(相補鎖も)を書き出す。 5-CGTCGTTTTACAACGTCGTGACIGAAAACCCTGGCGTTACCCAAC-3' 3-GCAGCAAAATGTTCGAGCACTG|CTTTTGGGACCGCAATGGGTTG-5 オーバーラップ領域を15 base 選定する。 (変異部分をなるべく中心にする) 5-CGTCGTTTTACAACGTCGTGAC|GAAAACCCTGGCGTTACCCAAC-3' 3-GCAGCAAAATGTTCGAGCACTG|CTTTTGGGACCGCAATGGGTTG-5' * 変異部分 から 18 base 3" 側に伸ばす。 5-CGTCGTTTTACAACGTCGTGAC|GAAAACCCTGGCGTTACCCAAC-3' 3-GCAGCAAAATGTTCGAGCACTG|てTTTTGGGACてGてAATGGGTTG-5' 部分を削除して、プライマー配列とする 5-TCGTGAC「GAAAACCCTGGCGTTACC-3" プライマー内部の塩基配列を変えることで、終止コドン 挿入やアミノ酸の置換、欠失や挿入が可能 A) 置換 3-CAAAATGTTCGAGCACTG|TTTTTGGG-5" *欠失のサイズに制限はありません。 タカラバイオのキットのマニュアルより 図3B. 欠失変異のためのプライマー設計 GAC」の部分を TGA に置換する場合 C) 挿入 5-CGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAAC-3' 3-GCAGCAAAATGTTCGAGCACTGACCCTTTTGGGACCGCAATGGGTTG-5' * 置換を行う |の部分に(CTCGAG)を挿入する場合 5-CGTCGTTTTACAACGTCGTTGATGGGAAAACCCTGGCGTTACCCAAC-3' 3-GCAGCAAAATGTTCGAGCAACT|ACCCTTTTGGGACCGCAATGGGTTG-5' GGGAAAACCCTGGCGTTACCCAAC-3" 5-CGTCGTTTTACAACGTCGTGAC1 3-GCAGCAAAATGTTCGAGCACTGA|CCCTTTTGGGACCGCAATGGGTTG-5' オーバーラップ領域を15 base 選定する。 (変異部分をなるべく中心にする) * Insertion を行う 5-CGTCGTTTTACAACGTCGTTGATGGGAAAACCCTGGCGTTACCCAAC-3' 3-GCAGCAAAATGTTCGAGCAACT|ACCCTTTTGGGACCGCAATGGGTTG-5' 5-CGTCGTTTTACAACGTCGTGACTCTCGAGGGGAAAACCCTGGCGTTACCCAAC-3' 3-GCAGCAAAATGTTCGAGCACTGAGAGCTC|CCCTTTTGGGACCGCAATGGGTTG-5' * 変異部分 口から 18base 3"側に伸ばす オーバーラップ領域を15 base 選定する。 (変異部分をなるべく中心にする) 5-CGTCGTTTTACAACGTCGTTGATGGGAAAACCCTGGCGTTACCCAAC-3" 3-GCAGCAAAATGTTCGAGCAACTACCCTTTTGGGACCGCAATGGGTTG-5" 部分を削除して、プライマー配列とする 5-CGTCGTTTTACAACGTCGTGACTCTCGAGGGGAAAACCCCTGGCGTTACCCAAC-3' 3-GCAGCAAAATGTTCGAGCACTGAGAGCTC|CCCTTTTGGGACCGCAATGGGTTG-5" * 変異部分 から 18 base 3'側に伸ばす 5-CGTCGTTGATGGGAAAACCCTGGCGTT-3' 3-CAGCAAAATGTTCGAGCAACTACCCTT-5" 5-CGTCGTTTTACAACGTCGTGACTCTCGAGGGGAAAACCCTGGCGTTACCCAAC-3" 3-GCAGCAAAATGTTCGAGCACTGAGAGCTCてててTTTTGGGACCGCAATGGGTTG-5" *1~15塩基の置換が可能です。 部分を削除して、プライマー配列とする 図3A.置換変異のためのプライマー設計 5-GACTCTCGAGGGGAAAACCCTGGCGTTA-3' 3-AAAATGTTCGAGCACTGAGAGCTCてててTT-S" *1~15塩基の挿入が可能です。 この場合は終止コドンだが、任意のコドンに変換して 対応するアミノ酸に置換することが可能 図3C. 挿入変異プライマーの設計 課題0-5 図AのGACはASP(アスパラギン酸)のコドンである。ここを下記のアミノ酸に置換する時の塩基配列を記せ 1) Met、2)Trp、3) His (2種類あり) コドン表は第8回の資料を参照し、 DNA配列で回答すること(UはT)。

回答募集中 回答数: 0
英語 高校生

プロミネンス1のLesson8のExercisesです。全然わからないです😭至急お願いします🙇🏻‍♀️🙇🏻‍♀️🙇🏻‍♀️🙇🏻‍♀️🙇🏻‍♀️

The State-of-the-Art Origami Engineering 5of the Exercises hange the word in parentheses into the appropriate form. 1(See) the pictures in the album, I remembered my happy childhood.. 2(See) from here, Mt. Fuji looks really beautiful. 3.I called my uncle, (thank) him for the nice Christmas present. 4. My teacher was standing by the front gate, (look) straight at me. 5.(Be) busy with his studies, he can't find time to play sports. B Fill in the blanks so that the two sentences have almost the same meaning. 1. In fact, he was very sick. >( The )( fAct )was ( that ) he was very sick. 2. He came here to speak out against us and made nothing but trouble. > He came here to speak out against us, ( ) nothing but trouble. 3. If I hadn't seen it myself, I wouldn't have believed it. > Since I ( ) it myself, I ( )it. C Write in the missing words to complete the sentence. 1.私のおじは1人で家を建てた。 My uncle built a house on ( his )( 0wn ). 2.芸術は一握りの者のためだけにあるのではない。 Art is not ( ) the few. 3.真の解決策はより良いコミュニケーションにあるのかもしれない。 The real solution may ( ) better communication. 4.彼が手伝ってくれたら, 私たちはもっと早く仕事を終わらせられる。 ) to finish the job sooner. His help will ( D Put the words in the correct order to make a sentence. 1. The (don't /is/listen/that / trouble / you / you / with). 2. If (had / have /I/I/known, / told/you/ would). 3. This (your / be/cannot / case/ rule/ to / applied). 4. The (nothing/up/meeting / deciding / ended).

回答募集中 回答数: 0
英語 高校生

アから順番に12131であってますか? わかる方教えてください!! お願いします!

Earth has two sets of poles: the geographic poles and the magnetic poles. The geographic poles are located at the northemmost and southernmost ends of the earth. The location of the magnetic poles, on the other hand, are ( ア ) moving, This fact has several implications for navigation and transportation. Shifts in the position of the North Magnetic Pole are nothing new. Since 1831, scientists have been tracking its location. The movements are caused by changes in the flow of swirling molten iron in the earth's core which affect how the earth's magnetic field behaves. What is surprising to scientists is the speed at which the pole has been moving in recent years. For most of the 20th century, magnetic north was located around the northern parts of Canada. It drifted around, moving slowly north at an ( イ ) speed of approximately 10 kilometers per year. In recent decades, that rate has increased significantly to about 55 kilometers per year. The North Magnetic Pole is now moving away from Canada toward eastem Russia. While scientists can't fully explain how changes in the carth's molten core are affecting the pole's movement, they can map the earth's magnetic field. Doing so allows them to ( ウ) hé rate of change over time. This, in turn, provides information on how the magnetic field may shift in the future. Information on changes to the earth's magnetic field is used to produce the World Magnetic Model (WMM). This model is used as the basis for all fornms of modern navigation, from how ships move at sea to mapping software in smartphones. ( エ) in the model, caused by shifts in the real location of the North Magnetic Pole, can seriously impact almost all foms of modern transportation. The model is updated every five years, but in 2018, during routine checks of the 2015- 2020 model cycle, scientists noticed a problem. Because of the rapidly shifting pole, the WMM was close to being outside the acceptable limits for navigational errors. This forced the release of an updated version of the model、 It was revised ( オ ) in 2020, and the current version is expected to last until 2024, As well as keeping track of the WMM's accuracy, scientists continue investigating why the magnetic field is changing so dramatically. This essential work will ensure a safe journey for everyone navigating their way through the world.

未解決 回答数: 1
英語 高校生

ア順番に12131であってますか? わかる方教えてください!!!! お願いします!

For most of the 20th century, magnetic north was located around the northern parts of molten iron in the earth's core which affect how the earth's magnetic field behaves. What is surprising to scientists is the speed at which the pole has been moving in recent years. magnetic poles, on the other hand, are ( ア ) moving. This fact has several implications poles are located at the northernmost and southernmost ends of the earth. The location of the Shifts in the position of the North Magnetic Pole are nothing new. Since 1831, scientists Earth has two sets of poles: the geographic poles and the magnetic poles. The geographic have been tracking its location. The movements are caused by changes in the flow of swirling for navigation and transportation. Canada. It drified around, moving slowiy north at an ( イ ) speed of approximately 10 MIometers per year. In recent decades. that rate has increased significantly to about 3 KIIometers per year. The North Magnetic Pole is nowmoving away from Canada toward eastern Russia. While scientists can't fully explain how changes in the earth's molten core are affecting the pole's movement, they can map the earth's magnetic field. Doing so allows them to (ウ ) the rate of change over time. This, in turn, provides information on how the magnetic field may shift in the future. Information on changes to the earth's magnetic field is used to produce the World Magnetic Model (WMM). This model is used as the basis for all forms of modern navigation, from how ships move at sea to mapping software in smartphones. ( エ ) inthe model, caused by shits in the real location of the North Magnetic Pole, can seriously impact almost all forms of moderntransportation. The model is updated every five years, but in 2018, during routine checks of the 2015- 2020 model cycle, scientists noticed a problem. Because of the rapidly shifting pole, the WMM was close to being outside the acceptable limits for navigational errors. This forced the release of an updated version of the model. was revised ( オ ) in2020, and the current version is expected to last until 2024. As well as keeping track of the WMM's accuracy, scientists continue investigating why the magnetic ficld is changing so dramatically. This essential work will ensure a safe journey for everyone navigating their way through the world.

回答募集中 回答数: 0