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英語 高校生

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The authoe w wsu In the beginning I wrote my diary on the backs of paper place mats. My friend and 答問題) 口次の英文は、筆者が20歳の頃に書き始めた日記についての話である。英文を読んで、あ le I were hitchhiking at the time. I was mailing regular letters and posteards to my friends back home, but because I had no fixed address, no one could answer them との間いに答えよ。 (配点 40) d And so I began writing to myself. Those first several years are hard to reread, not Seven is trulya wonderful age. For two days. That's the length of time my friend because they're boring-a diary is fully licensed to be boring- but because the writne is so horribly *affected. Pam and her son, Tyler, who is in the second grade, normally visit. He's at the stage (注)*conviction =確信 *repository =宝庫 where whatever I do, he wants to do. This includes wearing button-down shirts; singing * devotion =専念, 献身 *affected =気取った the same song until everyone begs you to stop; and carrying a small reporter's notebook. I gave him one the last time he came to the house and, imitating me, he stuck it in his pocket alongside a pen. That afternoon my friend drove us to a nearby town. There was 番号で答えよ。 an issue of the local paper in the backseat of the car, and reading it on our way there, I 2 1 came upon a headline that read, "Dangerous Olives Could Be on Sale." “Hmm, I said, and I copied it into my littlenotebook. l Tyler did the same but with less *conviction. "Why are we doing this again?" “It's for your diary," I explained. “You write things down during the day, then v tomorrow morning you expand on them." 4 “But why?" he asked. “What's the point?" ャ 3 That's a question I've asked myself every day since September 5, 1977. I hadn't known on September 4 that the following afternoon I would start keeping a diary, or that it would consume me for the next thirty-five years and counting. It wasn't something Td been putting off, but once I began, I knew that I had to keep doing it. I knew as wel that what I was writing was not a journal but an old-fashioned, secret diary. Often the terms are used in almost the same way, though I've never understood o 問2 下線部(ア)の内容を具体的に日本語で説明せよ。 why. Both have the word "day" at their root, but a journal, in my opinion, is a d hio hi d *repository of ideas - your brain on the page. A diary, by contrast, is your heart. As for “journaling," a verb that appeared at around the same time as “scrapbooking," that just means you're strange and have way to0 much time on your hands. ontdo bd al o ed sw ai o A few things have changed since that first entry in 1977, but I've never hesitated in ld eo o botele d my "devotion, skipping, on average, maybe one or two days a year. It's not that I think v e sd olaon my life is important. Perhaps it just feeds into my compulsive nature, the need to do the e d ba l exact same thing at the exact same time every morning. Some diary sessions are longer than others, but the length has more to do with my mood than with what's going on. 間3 次の英文は、筆者の日記に対する考えをまとめたものである。英文の空所( O), (の)に入れるのに最も適当なものを,それぞれ下の1~4のうちから一つずつ選び、 問5 下線部(イ)の理由について、当時の筆者の行動とともに次のようにまとめたい。次の空 所に35字程度の日本語を補い。文を完成させよ。ただし、旬読点も字数に数える。 番号で答えよ。ただし、同じ番号を二度用いてはならない。 当時,筆者は( "Journal" and "diary," both come from the same word originally, but the former is a warehouse of ideas or( の )on the page, while the latter is( の 1 your brain 2 your heart 3 your letters 4 your terms 3odw d 開4 次の Question に対するAnswer となるように、空所に入れるのに適当な内容を、英語で 補え。 Ouestion:Why has the author written in his diary almost every day since 1977? Answer He has never hesitated to keep a diary because he might feel uneasy if he 問6 次の英文は本文全体の内容をまとめたものである。空所(①. ) ~ ( ① ) に入れ るのに最も適当なものを,それぞれ下の1~4のうちから一つずつ選び、番号で答えよ。 thinks a child of age seven will ( ①)anything adults do. When the uthor did something, his friend's son, Tyler, would do the same thing. However, Tyler had a(の) about why the author kept a diary. The author has been keeping his Taiary for a long time. The contents of the first several years, however, are too affected for him to ( @ ) again. の 1 ak 2 Copy の 1 bellef 3 keep 2 confidence 4 1 『ead 3 eにTel 2 ing 4 question 3 underutand Write

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生物 高校生

この問題を教えてください!

課題 D-3 下の図を参考に次の文章の空欄を埋めよ 細胞膜はタンパク質を通さない。そこでタンパク質を分泌するためには(① )と呼ばれ る特別な装置が必要である。分泌されるタンパク質には( 12 )に( 13 )ペプチドがつ いた形で翻訳される。これは分必される際に膜上の( 0 )によって切断され、( 5 )タ ンパク質となる。 PET226は( 16 )という®を付加できるようになっており、タグは(① ) に付加できるようになっている。 大腸菌で大量にタンパク質を作らせると、( 1® )を形成することがある。これはタンパク 質の正しい折りたたみ( 19 )が追いつかないことが主な原因である。そこで、生物が本 来持っている、折りたたみを補助するタンパク質群である( 20 )を増強した大腸菌を用 いることで、回避できる可能性がある。 PET226 マルチクリーニングサイト周辺 T7 promoter primer #69348-3 Bgl II T7 promoter lac operator Xbal rbs BSPMI pelB leader MscI Ncol Ndel BamHI EcoRI Sacl MetLysTyrLeuLeuProThrAlaAlaAlaGlyLeuLouLeuLeuAlaAlaGInProAlaMetAlqMetAsp1leGlyIleAsnSerAspProAsnSerSerSer Sall Hind II Eag」 Not」 Aval Xhol His-Tag signal peptidase Bpu11021 ValAspLysLeuAlaAlaAlaLeuGluHIsHisHisHIsHIsHisEnd T7 terminator T7 terminator primer #69337-3

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生物 高校生

教えて欲しいです

課題 D-2 下の図を参考に次の文章の空欄を埋めよ 大腸菌で組換えタンパク質を生産させる時には、大腸菌由来のタンパク質を取り除 き、目的のタンパク質を(① )する必要がある。そのために目的タンパク質に付加 されるのが(2 )と呼ばれるアミノ酸配列である。 市販の大腸菌用( 3 )ベクターであるPET156では、目的タンパク質遺伝子である インサートの(4 )コドンを( 5 )のATGに合わせて挿入することで、が目的タン パク質の(6)末端に( ⑦ )タンパク質として、ニッケルイオンと相互作用する (His)6 配列が付加される。精製はゲル担体に固定された( ® )との( 9 )を利用 して行う。また、融合部にはトロンビンなどの認識部位が挿入されており、精製後、 当該( 0 )処理により、2を除けるように設計されている。 PET156 マルチクリーニングサイト周辺 T7 promoter primer #69348-3 T7 promoter lac operator rbs Bgl|| AGATCTCGATCCCCCGAAATTAATACCACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAG/ Xbal Ncol His-Tag Nde l Xhol BamHI HetGlySerSerHIisHIsHI aHIsHIaHI aSerSerGlyLouVaiProArgGlySerHIsHetLcuGluAspProAlaAlaAsnLysAlaArg Bpu1102| thrombin T7 terminator AAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTIG LysGluAlaGluLcuAlaAlaAlaThrAlaGluGInEnd T7 terminator primer #69337-3

解決済み 回答数: 1