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生物 高校生

PCR法の問題です。 3サイクル目に入ると、700とか900とかの塩基が出てくる気がするんですが、なぜnサイクル目には1000塩基より小さい塩基がないのでしょうか? いろいろ調べたのですがわかりません。よろしくお願いします。

第1章 B 18 PCR法 その2 ** 下図の左側に示すような1,400 塩基対のDNA分子の中に存在するある遺伝子を, 長さ20塩基のプライマーFと長さ21 塩基のプライマー R を用いて PCR法にて増 幅することにした。プライマーFの5′末端は鋳型DNAの300塩基内側に,プライマー Rの5 末端は鋳型 DNA の 100 塩基内側に結合する。下図の右側には第1サイク ルの途中までの様子が示してある。PCR 反応開始時の反応チュープ中には,1,400 塩基対の鋳型の2本鎖DNA が1分子,耐熱性の DNAポリメラーゼ,それぞれの プライマー,4種類のヌクレオチドが,増幅に最適化された反応液に入っていると する。反応中に,2種類のプライマーと4種類のヌクレオチドは枯渇せず,DNA ポ しっかつ リメラーゼは失活しないとする。 PCR反応は以下のサイクルで行い, 理想的な条件 下で行われるとする。 サイクル 95℃に加熱し1分間保温する。 を用 55℃に冷やし1分間保温する。 72℃に加熱し2分間保温する。 5' 13 1,400塩基 1,400塩基 15' 35 のじ ■3'′ → 5′ 13' ↑300塩基 プライマー F- プライマー R 100塩基 3' 15' 3'D 問1 第1サイクル終了後,どのような長さのDNAが何分子,反応液中にあ るか,以下の例にならって答えよ。 ただし,対象とするDNAは,長さが 100 塩基以上のものとし,1本鎖DNA として何分子あるかを答えよ。 例:1,400 塩基の DNA が 10 分子,1,500 塩基のDNAが2分子 問2 第2サイクル終了後は,どのような長さのDNA が何分子,反応液中に あるか。 問1の答え方にならって答えよ。 問3 □サイクル終了後は,どのような長さのDNA が何分子,反応液中にあ るか。 問1の答え方にならって答えよ。 問4 耐熱性のDNAポリメラーゼは、どのような生物に由来する酵素か。 側内 [兵庫医大〕

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英語 中学生

添削お願いします🙇🏻‍♀️՞ 写真は左から、原文、問題、自分の解答です。 模範解答は、 D.Why don't you ask my mother and grand mother? E.They will tell you more about my red kimono... 続きを読む

(Nana is showing Kate a photo at home.) Kate: You are wearing a red kimono in this photo. Nana: Thank you. My mother took it at my uncle's wedding. Kate: The flower pattern on your kimono is amazing. Nana: That's true. It's my family's precious kimono. Kate: Why is the kimono precious? Nana: Actually, is bought my grandmother I this the kimono ] for my mother thirty years ago. Kate: Oh, you used your mother's kimono. Nana: Yes, but she gave it to me last year. So the kimono is ( @). Kate: Why did your mother give it to you? Nana: This red kimono has long sleeves. She thinks this kind of kimono is for young people, so she doesn't wear it now. Kate: I have a ( ℗ ) experience. My mother has a nice dress in her closet, but she doesn't wear it. I always wear it when I go to birthday parties. Nana: I'm sure your friends like the dress. Kate: Thanks. When I wear it, ⠀ Nana: : The designs of old clothes are different from the new ones, right? み Kate: Yes! I think wearing used clothes is fun. ( © ), wearing other people's clothes isn't easy because of the size. Actually, my mother's dress was large for me, so she adjusted it. Who adjusted your kimono? Nana: B Sonimom vis ns diwalls of WH Kimono has a simple shape, so it can be used easily by different people. Kate: Interesting. Kimono is not only beautiful but also functional. Nana: Right, so I love kimono. I'm glad to give my red kimono a new life. Kate: C Nana: If I wear my red kimono, it will have more chances to get out of the closet like your mother's dress. Kate: That's a good idea to use the kimono again. smozgnilos ayoung H Nana: I'll wear it on special days!

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英語 中学生

この問題の(1)→remind recollect rememberの違いについて解説して欲しく (2)何が対象のcoverか について詳しく教えて欲しいです。二枚目の選択肢にピンク丸をつけたものが正解です。

Test 1 READING AND USE OF ENGLISH Part 1 For questions 1-8, read the text below and decide which answer (A, B, C or D) best fits each gap. There is an example at the beginning (0). Mark your answers on the separate answer sheet. 12 33 4 Example: 0 A gather C find B produce D gain A B Alfred Wainwright Alfred Wainwright came from a relatively poor family but managed to (0) ...ain qualifications in 鍼 accountancy. However it is not for his skill in accountancy that he is (1) yemem but for his pictorial guidebooks to the English Lake District. bered The Lake District is in the north-west of England and (2) Covers, an area of some 2,292 square kilometres. As its name (3) implies, it is an area of lakes and mountains. Alfred first went there on a walking holiday in 1930 and immediately fell in love with the area. He (4) divided the Lake District into seven parts and wrote a guide for each of them. The guides (5) consist entirely of copies of his hand-written manuscripts. All have descriptions of walks with hand-drawn maps and sketches of views from the summits of the different mountains. He intended the books to be just for his own personal (6) use... but was eventually (7) publish them. They are beautiful books which (8) ........ remain as popular as ever. to persuaded 5 276

解決済み 回答数: 1