至急です。中3模試です 1部しか載せられずすみません。 並び替えの部分を適切に並び替える問題なのですが、 It was his first time to tryが答えで、 his が何故ここにはいるのか分かりません。教えて頂きたいです。 また、出来れば長文の中にある並び替... 続きを読む

*ヘTTTOTT Last week, (when I was searching the Internet, I found something vC1 engineer in *Sweden, Ms. Hertz, invented a *device for elderly people. I watched a video about it. It's a *combination of an *exercise bike and a large *domed 、screen. 3 The domed screen displays *images of streets and buildings,In the video, a 75 -year-old man sat on the bike and “visited" his hometown. O1 [ try / time / his / was (it/ to / first] the device. Che man had dementia and had memory_ problems. His wife said, "It's hard for us to talk about our memories. He has forgotten a lot." She anda staff member were standing by him. When he started *pedaling, the *view on the screen in front of him started to It was almost like *virtual reality/but) he didn't need the *goggles, and could share move. the view with others. When he saw one building, he said, “I first met my wife here. She was beautiful," “Oh, you remember that!" his wife said and looked very happy. He visited *familiar nlaces and talked about his memories.I remembered Ms. Nakai then.

教えて欲しいです

課題 D-2 下の図を参考に次の文章の空欄を埋めよ 大腸菌で組換えタンパク質を生産させる時には、大腸菌由来のタンパク質を取り除 き、目的のタンパク質を(① )する必要がある。そのために目的タンパク質に付加 されるのが(2 )と呼ばれるアミノ酸配列である。 市販の大腸菌用( 3 )ベクターであるPET156では、目的タンパク質遺伝子である インサートの(4 )コドンを( 5 )のATGに合わせて挿入することで、が目的タン パク質の(6)末端に( ⑦ )タンパク質として、ニッケルイオンと相互作用する (His)6 配列が付加される。精製はゲル担体に固定された( ® )との( 9 )を利用 して行う。また、融合部にはトロンビンなどの認識部位が挿入されており、精製後、 当該( 0 )処理により、2を除けるように設計されている。 PET156 マルチクリーニングサイト周辺 T7 promoter primer #69348-3 T7 promoter lac operator rbs Bgl|| AGATCTCGATCCCCCGAAATTAATACCACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAG/ Xbal Ncol His-Tag Nde l Xhol BamHI HetGlySerSerHIisHIsHI aHIsHIaHI aSerSerGlyLouVaiProArgGlySerHIsHetLcuGluAspProAlaAlaAsnLysAlaArg Bpu1102| thrombin T7 terminator AAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTIG LysGluAlaGluLcuAlaAlaAlaThrAlaGluGInEnd T7 terminator primer #69337-3

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127.… remain several important issues to resolve, but the negotiations appear to be moving toward a successful conclusion. (A) It (B) She (C) They (D) There 130. Sometimes negotiations break down for unexpected reasons, even when the two sides have respeet for one …。 (A) other (B) the other (C) others (D) another 134. In the early 1990s, the Japanese economy struggled under a heavy bad debt burden, with the additional problem of slow economic growth. (C) on (A) along (B) in (D) out 135.… thorough market research, it is difficult for a manufacturer to determine how well-known its brand name is, and how people will react to different style changes. (A) Unless (B) Without (C) Other than (D) Outside of the next town for another hour or so. (C) get 101. The train won't (A) arrive (B) come (D) reach

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125. A lack of long-term planning contributed into trouble sooner or later. (A) to -a sense that the company would run (B) with (C) for (D) at 127.…… remain several important issues to resolve, but the negotiations appear to be moving towarda successful conclusion. (A) It (B) She (C) They (D) There 130. Sometimes negotiations break down for unexpected reasons, even when the two sides have respect for one -…。 (A) other (B) the other (C) others (D) another 134. In the early 1990s, the Japanese economy struggled under a heavy bad debt burden, …with the additional problem of slow economic growth. (A) along (B) in (C) on (D) out 135.… thorough market research, it is difficult for a manufacturer to determine how well-known its brand name is, and how people will react to different style changes. (A) Unless (B) Without (C) Other than (D) Outside of 101. The train won't … the next town for another hour or so. (B) come (A) arrive (C) get (D) reach

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130. Nancy almost never asks for help - her work, but last week she was so overwhelmed that she did. (A) with (B) for (C) at (D) to 131. Instead of buying a car, you might want to try leasing one by ……… (A) the month. (B) a (C) its (D) this 133. Resumes with cover letters may be submitted by fax, e-mail or conventional mail, but initial inquiries may not be made in -… or by telephone. (A) oneself (B) person (C) self (D) them 107. The thief came out the front door of the liquor store and walked off that direction. (A) at (B) on (C) in (D) to 109. No one is allowed to enter the secret facility without presenting two …… of photographic identification. (A) pieces (B) acts (C) marks (D) parts 125. A lack of long-term planning contributed a sense that the company would run into trouble sooner or later. (A) to (B) with (C) for (D) at 127.…… remain several important issues to resolve, but the negotiations appear to be moving toward a successful conclusion. (A) It (B) She (C) They (D) There 130. Sometimes negotiations break down for unexpected reasons, even when the two sides have respect for one ……。 (A) other (B) the other (C) others (D) another 134. In the early 1990s, the Japanese economy struggled under a heavy bad debt burden, …with the additional problem of slow economic growth. (A) along (B) in (C) on (D) out 135.… thorough market research, it is difficult for a manufacturer to determine how well-known its brand name is, and how people will react to different style changes. (A) Unless (B) Without (C) Other than (D) Outside of 101. The train won't -- the next town for another hour or so. (B) come (A) arrive (C) get (D) reach

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……… her work, but last week she was so 130. Nancy almost never asks for help overwhelmed that she did. (A) with (B) for (C) at (D) to 131. Instead of buying a car, you might want to try leasing one by --month. (A) the (B) a (C) its (D) this 133. Resumes with cover letters may be submitted by fax, e-mail or conventional mail, but initial inquiries may not be made in or by telephone. (A) oneself (B) person (C) self (D) them 107. The thief came out the front door of the liquor store and walked off that direction. (A) at (B) on (C) in (D) to 109. No one is allowed to enter the secret facility without presenting two -…of photographic identification. (A) pieces (B) acts (C) marks (D) parts 125. A lack of long-term planning contributed a sense that the company would run into trouble sooner or later. (A) to (B) with (C) for (D) at 127.……… remain several important issues to resolve, but the negotiations appear to be moving toward a successful conclusion. (A) It (B) She (C) They (D) There 130. Sometimes negotiations break down for unexpected reasons, even when the two sides have respect for one …. (A) other (B) the other (C) others (D) another 134. In the early 1990s, the Japanese economy struggled under a heavy bad debt burden, … with the additional problem of slow economic growth. (A) along (B) in (C) on (D) out 135.……… thorough market research, it is difficult for a manufacturer to determine how well-known its brand name is, and how people will react to different style changes. (A) Unless (B) Without (C) Other than (D) Outside of 101. The train won't ……… the next town for another hour or so. (B) come (A) arrive (C) get (D) reach

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130. Nancy almost never asks for help … overwhelmed that she did. (A) with her work, but last week she was so (B) for (C) at (D) to 131. Instead of buying a car, you might want to try leasing one by month. (A) the (B) a (C) its (D) this 133. Resumes with cover letters may be submitted by fax, e-mail or conventional mail, but initial inquiries may not be made in -- or by telephone. (A) oneself (B) person (C) self (D) them 107. The thief came out the front door of the liquor store and walked off direction. (A) at that (B) on (C) in (D) to 109. No one is allowed to enter the secret facility without presenting two -…of photographic identification. (A) pieces (B) acts (C) marks (D) parts 125. A lack of long-term planning contributed a sense that the company would run into trouble sooner or later. (A) to (B) with (C) for (D) at 127.…… remain several important issues to resolve, but the negotiations appear to be moving toward a successful conclusion. (A) It (B) She (C) They (D) There 130. Sometimes negotiations break down for unexpected reasons, even when the two sides have respect for one ……。 (A) other (B) the other (C) others (D) another 134. In the early 1990s, the Japanese economy struggled under a heavy bad debt burden, … with the additional problem of slow economic growth. (A) along (B) in (C) on (D) out 135.… thorough market research, it is difficult for a manufacturer to determine how well-known its brand name is, and how people will react to different style changes. (A) Unless (B) Without (C) Other than (D) Outside of 101. The train won't …… the next town for another hour or so. (B) come (A) arrive (C) get (D) reach

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130. Nancy almost never asks for help her work, but last week she was so overwhelmed that she did. (A) with (B) for (C) at (D) to 131. Instead of buying a car, you might want to try leasing one by month. (A) the (B) a (C) its (D) this 133. Resumes with cover letters may be submitted by fax, e-mail or conventional mail, but initial inquiries may not be made in ……………or by telephone. (A) oneself (B) person (C) self (D) them 107. The thief came out the front door of the liquor store and walked off that direction. (A) at (B) on (C) in (D) to 109. No one is allowed to enter the secret facility without presenting two - …of photographic identification. (A) pieces (B) acts (C) marks (D) parts 125. A lack of long-term planning contributed …a sense that the company would run into trouble sooner or later. (A) to (B) with (C) for (D) at 127.………… remain several important issues to resolve, but the negotiations appear to be moving toward a successful conclusion. (A) It (B) She (C) They (D) There 130. Sometimes negotiations break down for unexpected reasons, even when the two sides have respect for one -…。 (A) other (B) the other (C) others (D) another 134. In the early 1990s, the Japanese economy struggled under a heavy bad debt burden, … with the additional problem of slow economic growth. (A) along (B) in (C) on (D) out 135.………… thorough market research, it is difficult for a manufacturer to determine how well-known its brand name is, and how people will react to different style changes. (A) Unless (B) Without (C) Other than (D) Outside of 101. The train won't the next town for another hour or so. (A) arrive (B) come (C) get (D) reach

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130. Nancy almost never asks for help her work, but last week she was so overwhelmed that she did. (A) with (B) for (C) at (D) to 131. Instead of buying a car, you might want to try leasing one by --month. (A) the (B) a (C) its (D) this 133. Resumes with cover letters may be submitted by fax, e-mail or conventional mail, but initial inquiries may not be made in or by telephone. (A) oneself (B) person (C) self (D) them 107. The thief came out the front door of the liquor store and walked off that direction. (A) at (B) on (C) in (D) to 109. No one is allowed to enter the secret facility without presenting two - …of photographic identification. (A) pieces (B) acts (C) marks (D) parts 125. A lack of long-term planning contributed a sense that the company would run into trouble sooner or later. (A) to (B) with (C) for (D) at 127.…… remain several important issues to resolve, but the negotiations appear to be moving toward a successful conclusion. (A) It (B) She (C) They (D) There 130. Sometimes negotiations break down for unexpected reasons, even when the two sides have respect for one…………。 (A) other (B) the other (C) others (D) another 134. In the early 1990s, the Japanese economy struggled under a heavy bad debt burden, …with the additional problem of slow economic growth. (A) along (B) in (C) on (D) out 135.… thorough market research, it is difficult for a manufacturer to determine how well-known its brand name is, and how people will react to different style changes. (A) Unless (B) Without (C) Other than (D) Outside of 101. The train won't the next town for another hour or so. (A) arrive (B) come (C) get (D) reach

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130. Nancy almost never asks for help - her work, but last week she was so overwhelmed that she did. (A) with (B) for (C) at (D) to 131. Instead of buying a car, you might want to try leasing one by month. (A) the (B) a (C) its (D) this 133. Resumes with cover letters may be submitted by fax, e-mail or conventional mail, but initial inquiries may not be made in ………or by telephone. (A) oneself (B) person (C) self (D) them 107. The thief came out the front door of the liquor store and walked off direction. (A) at that (B) on (C) in (D) to 109. No one is allowed to enter the secret facility without presenting two - …of photographic identification. (A) pieces (B) acts (C) marks (D) parts 125. A lack of long-term planning contributed …a sense that the company would run into trouble sooner or later. (A) to (B) with (C) for (D) at 127.…… remain several important issues to resolve, but the negotiations appear to be moving toward a successful conclusion. (A) It (B) She (C) They (D) There 130. Sometimes negotiations break down for unexpected reasons, even when the two sides have respect for one………。 (A) other (B) the other (C) others (D) another 134. In the early 1990s, the Japanese economy struggled under a heavy bad debt burden, …with the additional problem of slow economic growth. (A) along (B) in (C) on (D) out 135.… thorough market research, it is difficult for a manufacturer to determine how well-known its brand name is, and how people will react to different style changes. (A) Unless (B) Without (C) Other than (D) Outside of 101. The train won't the next town for another hour or so. (A) arrive (B) come (C) get (D) reach